10.1184/R1/6097478.v1 Michael J. Higley Michael J. Higley Aryn Gittis Aryn Gittis Ian H. Oldenburg Ian H. Oldenburg Nina Balthasar Nina Balthasar Rebecca P. Seal Rebecca P. Seal Robert H. Edwards Robert H. Edwards Bradford B. Lowell Bradford B. Lowell Anatol C. Kreitzer Anatol C. Kreitzer Bernardo L. Sabatini Bernardo L. Sabatini Cholinergic interneurons mediate fast VGluT3-dependent glutamatergic transmission in the striatum. Carnegie Mellon University 2011 Amino Acid Transport Systems Acidic Animals Central Nervous System Stimulants Corpus Striatum Evoked Potentials Female Interneurons Male Mecamylamine Mice Picrotoxin Receptors Cholinergic N-Methyl-D-Aspartate Rhodopsin Scopolamine Hydrobromide 2011-01-01 00:00:00 Journal contribution https://kilthub.cmu.edu/articles/journal_contribution/Cholinergic_interneurons_mediate_fast_VGluT3-dependent_glutamatergic_transmission_in_the_striatum_/6097478 <p>The neurotransmitter glutamate is released by excitatory projection neurons throughout the brain. However, non-glutamatergic cells, including cholinergic and monoaminergic neurons, express markers that suggest that they are also capable of vesicular glutamate release. Striatal cholinergic interneurons (CINs) express the Type-3 vesicular glutamate transporter (VGluT3), although whether they form functional glutamatergic synapses is unclear. To examine this possibility, we utilized mice expressing Cre-recombinase under control of the endogenous choline acetyltransferase locus and conditionally expressed light-activated Channelrhodopsin2 in CINs. Optical stimulation evoked action potentials in CINs and produced postsynaptic responses in medium spiny neurons that were blocked by glutamate receptor antagonists. CIN-mediated glutamatergic responses exhibited a large contribution of NMDA-type glutamate receptors, distinguishing them from corticostriatal inputs. CIN-mediated glutamatergic responses were insensitive to antagonists of acetylcholine receptors and were not seen in mice lacking VGluT3. Our results indicate that CINs are capable of mediating fast glutamatergic transmission, suggesting a new role for these cells in regulating striatal activity.</p>