10.1184/R1/6104222.v1
Edward Horsey
Edward
Horsey
Jelena Jakovljevic
Jelena
Jakovljevic
Tiffany D. Miles
Tiffany D.
Miles
Piyanun Harnpicharnchai
Piyanun
Harnpicharnchai
John Woolford
John
Woolford
Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation.
Carnegie Mellon University
2004
Amino Acid Sequence
Cell Nucleolus
Molecular Sequence Data
Nuclear Proteins
RNA Precursors
RNA
Ribosomal
Ribosomes
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Sequence Alignment
2004-05-01 00:00:00
Journal contribution
https://kilthub.cmu.edu/articles/journal_contribution/Role_of_the_yeast_Rrp1_protein_in_the_dynamics_of_pre-ribosome_maturation_/6104222
<p>The Saccharomyces cerevisiae gene RRP1 encodes an essential, evolutionarily conserved protein necessary for biogenesis of 60S ribosomal subunits. Processing of 27S pre-ribosomal RNA to mature 25S rRNA is blocked and 60S subunits are deficient in the temperature-sensitive rrp1-1 mutant. We have used recent advances in proteomic analysis to examine in more detail the function of Rrp1p in ribosome biogenesis. We show that Rrp1p is a nucleolar protein associated with several distinct 66S pre-ribosomal particles. These pre-ribosomes contain ribosomal proteins plus at least 28 nonribosomal proteins necessary for production of 60S ribosomal subunits. Inactivation of Rrp1p inhibits processing of 27SA(3) to 27SB(S) pre-rRNA and of 27SB pre-rRNA to 7S plus 25.5S pre-rRNA. Thus, in the rrp1-1 mutant, 66S pre-ribosomal particles accumulate that contain 27SA(3) and 27SB(L) pre-ribosomal RNAs.</p>