10.1184/R1/6104222.v1 Edward Horsey Edward Horsey Jelena Jakovljevic Jelena Jakovljevic Tiffany D. Miles Tiffany D. Miles Piyanun Harnpicharnchai Piyanun Harnpicharnchai John Woolford John Woolford Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation. Carnegie Mellon University 2004 Amino Acid Sequence Cell Nucleolus Molecular Sequence Data Nuclear Proteins RNA Precursors RNA Ribosomal Ribosomes Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Sequence Alignment 2004-05-01 00:00:00 Journal contribution https://kilthub.cmu.edu/articles/journal_contribution/Role_of_the_yeast_Rrp1_protein_in_the_dynamics_of_pre-ribosome_maturation_/6104222 <p>The Saccharomyces cerevisiae gene RRP1 encodes an essential, evolutionarily conserved protein necessary for biogenesis of 60S ribosomal subunits. Processing of 27S pre-ribosomal RNA to mature 25S rRNA is blocked and 60S subunits are deficient in the temperature-sensitive rrp1-1 mutant. We have used recent advances in proteomic analysis to examine in more detail the function of Rrp1p in ribosome biogenesis. We show that Rrp1p is a nucleolar protein associated with several distinct 66S pre-ribosomal particles. These pre-ribosomes contain ribosomal proteins plus at least 28 nonribosomal proteins necessary for production of 60S ribosomal subunits. Inactivation of Rrp1p inhibits processing of 27SA(3) to 27SB(S) pre-rRNA and of 27SB pre-rRNA to 7S plus 25.5S pre-rRNA. Thus, in the rrp1-1 mutant, 66S pre-ribosomal particles accumulate that contain 27SA(3) and 27SB(L) pre-ribosomal RNAs.</p>