10.1184/R1/6104228.v1
Gisela Pöll
Gisela
Pöll
Tobias Braun
Tobias
Braun
Jelena Jakovljevic
Jelena
Jakovljevic
Andreas Neueder
Andreas
Neueder
Steffen Jakob
Steffen
Jakob
John Woolford
John
Woolford
Herbert Tschochner
Herbert
Tschochner
Philipp Milkereit
Philipp
Milkereit
rRNA maturation in yeast cells depleted of large ribosomal subunit proteins.
Carnegie Mellon University
2009
Blotting
Northern
Cytoplasm
Endonucleases
Models
Molecular
Mutation
RNA 3' End Processing
RNA Precursors
RNA Transport
RNA
Ribosomal
5.8S
Ribosome Subunits
Large
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
2009-01-01 00:00:00
Journal contribution
https://kilthub.cmu.edu/articles/journal_contribution/rRNA_maturation_in_yeast_cells_depleted_of_large_ribosomal_subunit_proteins_/6104228
<p>The structural constituents of the large eukaryotic ribosomal subunit are 3 ribosomal RNAs, namely the 25S, 5.8S and 5S rRNA and about 46 ribosomal proteins (r-proteins). They assemble and mature in a highly dynamic process that involves more than 150 proteins and 70 small RNAs. Ribosome biogenesis starts in the nucleolus, continues in the nucleoplasm and is completed after nucleo-cytoplasmic translocation of the subunits in the cytoplasm. In this work we created 26 yeast strains, each of which conditionally expresses one of the large ribosomal subunit (LSU) proteins. In vivo depletion of the analysed LSU r-proteins was lethal and led to destabilisation and degradation of the LSU and/or its precursors. Detailed steady state and metabolic pulse labelling analyses of rRNA precursors in these mutant strains showed that LSU r-proteins can be grouped according to their requirement for efficient progression of different steps of large ribosomal subunit maturation. Comparative analyses of the observed phenotypes and the nature of r-protein-rRNA interactions as predicted by current atomic LSU structure models led us to discuss working hypotheses on i) how individual r-proteins control the productive processing of the major 5' end of 5.8S rRNA precursors by exonucleases Rat1p and Xrn1p, and ii) the nature of structural characteristics of nascent LSUs that are required for cytoplasmic accumulation of nascent subunits but are nonessential for most of the nuclear LSU pre-rRNA processing events.</p>