10.1184/R1/6104564.v1 Li Liu Li Liu Qing Ye Qing Ye Yi-jen L. Wu Yi-jen L. Wu Wen-Yuan Hsieh Wen-Yuan Hsieh Chih-Lung Chen Chih-Lung Chen Hsin-Hsin Shen Hsin-Hsin Shen Shian-Jy Wang Shian-Jy Wang Haosen Zhang Haosen Zhang T. Kevin Hitchens T. Kevin Hitchens Chien Ho Chien Ho Tracking T-cells in vivo with a new nano-sized MRI contrast agent. Carnegie Mellon University 2012 Animals Cell Tracking Contrast Media Ferric Compounds Heart-Lung Transplantation Humans Jurkat Cells Magnetic Resonance Imaging Magnetite Nanoparticles Male Polyethylene Glycols Rats Regenerative Medicine T-Lymphocytes 2012-11-01 00:00:00 Journal contribution https://kilthub.cmu.edu/articles/journal_contribution/Tracking_T-cells_in_vivo_with_a_new_nano-sized_MRI_contrast_agent_/6104564 <p>UNLABELLED: Non-invasive in vivo tracking of T-cells by magnetic resonance imaging (MRI) can lead to a better understanding of many pathophysiological situations, including AIDS, cancer, diabetes, graft rejection. However, an efficient MRI contrast agent and a reliable technique to track non-phagocytic T-cells are needed. We report a novel superparamagnetic nano-sized iron-oxide particle, IOPC-NH2 series particles, coated with polyethylene glycol (PEG), with high transverse relaxivity (250 s(-1) mM(-1)), thus useful for MRI studies. IOPC-NH2 particles are the first reported magnetic particles that can label rat and human T-cells with over 90% efficiency, without using transfection agents, HIV-1 transactivator peptide, or electroporation. IOPC-NH2 particles do not cause any measurable effects on T-cell properties. Infiltration of IOPC-NH2-labeled T-cells can be detected in a rat model of heart-lung transplantation by in vivo MRI. IOPC-NH2 is potentially valuable contrast agents for labeling a variety of cells for basic and clinical cellular MRI studies, e.g., cellular therapy.</p> <p>FROM THE CLINICAL EDITOR: In this study, a novel PEG coated superparamagnetic nano-sized iron-oxide particle was investigated as a T-cell labeling agent for MRI studies. The reported particles can label T-cells with over 90% efficiency, without using transfection agents, HIV-1 transactivator peptide, or electroporation, therefore may enable more convenient preclinical call labeling studies.</p>