T cell signaling proteins 3D confocal microscope movies

dataset

posted on 2019-11-01, 21:28 authored by Robert MurphyRobert Murphy

This dataset contains 3D confocal microscope movies of T cell clones expressing different eGFP-tagged proteins as they undergo synapse formation with antigen presenting cells (APCs). Each movie shows a field of T cells and APCs every 20 s for 6 minutes after the cells are mixed. Each frame shows some cells in various stages of antigen presentation. The movies are for two parental T cell clones, four stimulation conditions and sixteen proteins. The acquisition and analysis of the movies is described in

D. J. Clark, L.E. McMillan, S.L. Tan, G. Bellomo, C. Massou, H. Thompson, L. Mykhaylechko, D. Alibhai, X. Ruan, K.L. Singleton, M. Du, A.J. Hedges, P.L. Schwartzberg, P. Verkade, R.F. Murphy, and C. Wülfing (2019) Transient protein accumulation at the center of the T cell antigen presenting cell interface drives efficient IL-2 secretion. eLife 8:e45789. https://doi.org/10.7554/eLife.45789

Funding

NSF Grants MCB1121793, MCB1121919, MCB1616492;

NIH Grant P41 GM103712

BBSRC BB/P011578/1

ERC PCIG11-GA-2012- 321554

History

Publisher Statement

The movies in this dataset were used in D. J. Clark, L.E. McMillan, S.L. Tan, G. Bellomo, C. Massou, H. Thompson, L. Mykhaylechko, D. Alibhai, X. Ruan, K.L. Singleton, M. Du, A.J. Hedges, P.L. Schwartzberg, P. Verkade, R.F. Murphy, and C. Wülfing (2019) Transient protein accumulation at the center of the T cell antigen presenting cell interface drives efficient IL-2 secretion. eLife 8:e45789. https://doi.org/10.7554/eLife.45789 The methodology for the analysis is described in K. T. Roybal, T. E. Buck, X. Ruan, B. H. Cho, D. J. Clark, R. Ambler, H. M. Tunbridge, J. Zhang, P. Verkade, C. Wülfing, and R. F. Murphy (2016) Computational spatiotemporal analysis identifies WAVE2 and Cofilin as joint regulators of costimulation-mediated T cell actin dynamics. Science Signaling 9:rs3. https://doi.org/10.1126/scisignal.aad4149

Date

2019-10-23

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Keywords

T cell activation confocal fluorescence microscopy gfp-tagging spatiotemporal modeling methods Signaling pathways immunological synapse antigen presenting cell (APC)

Licence

CC BY 4.0

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