Carnegie Mellon University
Browse

File(s) stored somewhere else

Please note: Linked content is NOT stored on Carnegie Mellon University and we can't guarantee its availability, quality, security or accept any liability.

Difference gel electrophoresis.

journal contribution
posted on 2009-06-01, 00:00 authored by Jonathan MindenJonathan Minden, Susan R. Dowd, Helmut E. Meyer, Kai Stühler

Difference gel electrophoresis (DIGE) was invented to circumvent the inherent variability of 2-DE. This variability is a natural consequence of separating thousands of proteins over a large space, such as a 15 x 20 cm slab of polyacrylamide gel. The originators of 2-DE envisioned being able to compare cancerous cells and normal cells to understand what makes these cells different. Gel-to-gel variability made this an extremely difficult task. We reasoned that if both samples could be run on the same gel, then the inherent variability would be obviated. Thus, we created matched sets of fluorescent dyes that allows one to compare two or three protein samples on a single gel. In the 12 years since the description of DIGE first appeared in Electrophoresis, this founding paper has been cited over 660 times. This review highlights some of the improvements and applications of DIGE. We hope these examples are illustrative of what has been done and where the field is headed.

History

Date

2009-06-01

Usage metrics

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC