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Fluorogen-activating proteins as biosensors of cell-surface proteins in living cells.
journal contributionposted on 2010-08-01, 00:00 authored by John Holleran, Dara Brown, Margaret H. Fuhrman, Sally A. Adler, Gregory FisherGregory Fisher, Jonathan JarvikJonathan Jarvik
This study explores the general utility of a new class of biosensor that allows one to selectively visualize molecules of a chosen membrane protein that are at the cell surface. These biosensors make use of recently described bipartite fluoromodules comprised of a fluorogen-activating protein (FAP) and a small molecule (fluorogen) whose fluorescence increases dramatically when noncovalently bound by the FAP (Szent-Gyorgyi et al., Nat Biotechnol 2010;00:000-000).
Adrenergic beta-2 Receptor AgonistsAnimalsBiosensing TechniquesCell MembraneCell SurvivalCystic Fibrosis Transmembrane Conductance RegulatorEndocytosisFluorescent DyesGlucose Transporter Type 4HumansMembrane ProteinsMiceMicroscopyFluorescenceNIH 3T3 CellsProtein StructureTertiaryReceptorsAdrenergicbeta-2