Carnegie Mellon University

File(s) stored somewhere else

Please note: Linked content is NOT stored on Carnegie Mellon University and we can't guarantee its availability, quality, security or accept any liability.

Genetically encoded pH sensor for tracking surface proteins through endocytosis.

journal contribution
posted on 2012-05-14, 00:00 authored by Anmol Grover, Brigitte SchmidtBrigitte Schmidt, Russell D. Salter, Simon C. Watkins, Alan WaggonerAlan Waggoner, Marcel BruchezMarcel Bruchez

Traffic cam: a tandem dye prepared from a FRET acceptor and a fluorogenic donor functions as a cell surface ratiometric pH indicator, which upon internalization serves to follow protein trafficking during endocytosis. This sensor was used to analyze agonist-dependent internalization of β(2)-adrenergic receptors. It was also used as a surrogate antigen to reveal direct surface-to-endosome antigen transfer between dendritic cells (not shown).