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Genetically encoded pH sensor for tracking surface proteins through endocytosis.
journal contributionposted on 2012-05-14, 00:00 authored by Anmol Grover, Brigitte SchmidtBrigitte Schmidt, Russell D. Salter, Simon C. Watkins, Alan WaggonerAlan Waggoner, Marcel BruchezMarcel Bruchez
Traffic cam: a tandem dye prepared from a FRET acceptor and a fluorogenic donor functions as a cell surface ratiometric pH indicator, which upon internalization serves to follow protein trafficking during endocytosis. This sensor was used to analyze agonist-dependent internalization of β(2)-adrenergic receptors. It was also used as a surrogate antigen to reveal direct surface-to-endosome antigen transfer between dendritic cells (not shown).