Carnegie Mellon University
Browse

STED nanoscopy in living cells using Fluorogen Activating Proteins.

journal contribution
posted on 2009-01-01, 00:00 authored by James Fitzpatrick, Qi Yan, Jochen J. Sieber, Marcus Dyba, Ulf Schwarz, Christopher Szent-GyorgyiChristopher Szent-Gyorgyi, Carol WoolfordCarol Woolford, Peter B. Berget, Alan WaggonerAlan Waggoner, Marcel BruchezMarcel Bruchez

We demonstrate the effectiveness of a genetically encoded Malachite Green (MG) binding fluorogen activating protein (FAP) for live cell stimulated emission depletion nanoscopy (STED). Both extracellular and intracellular FAPs were tested in living cells using fluorogens with either membrane expressed FAP or as an intracellular FAP-actin fusion. Structures with FWHM of 110-122nm were observed. Depletion data however suggests a resolution of 70nm with the given instrument.

History

Date

2009-01-01

Usage metrics

    Categories

    Keywords

    Biological SciencesBiological Sciences not elsewhere classified

    Licence

    In Copyright

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC