Carnegie Mellon University
Browse

Do Cheetos and Nacho Chips contain genetically modified DNA (GMOs)?

Download (12.49 MB)
poster
posted on 2024-06-28, 18:06 authored by Nour Nasrallah, Mariam Afifi

Genetically modified organisms play an important role in modern agriculture, yet concerns persist regarding their safety and labeling transparency. In this study, we aimed to detect the presence of genetically modified DNA in corn-based food products:
unlabeled nacho chips and puffed corn chips. We hypothesized successful detection of genes associated with genetic modifications in these products. DNA extraction from the food products was done first. Then, using single PCR, multiplex PCR, we targeted specific DNA markers, including CaMV35S, Zein (invertase) and Cry1Ab genes associated with GMOs. While positive controls showed expected gene expression patterns, no bands were observed in the food samples, indicating potential issues with DNA extraction and the need of repeating that step. Cry1Ab was also not detected in any sample which may suggest the need for PCR optimization. 

History

Date

2024-04-30

Academic Program

  • Biological Sciences

Advisor(s)

Annette Vincent Adviti Naik

Usage metrics

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC