Carnegie Mellon University
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Final poster-meeting of the minds spring'24 - Mariam Afifi.pdf (251.72 kB)

Functional Analysis of a novel lncRNA in Breast Cancer

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posted on 2024-06-28, 18:04 authored by Mariam Afifi

Non-coding RNAs (ncRNAs) make up most of the human transcriptome and have a role in gene regulation. Some long-ncRNAs (lncRNAs) can act as decoys or sponges for specific regulatory factors limiting their availability in the cell. Some examples of these regulatory factors are micro-RNAs (miRNAs) which act as negative regulators of mRNA. Cancer cells can specifically express decoys that sustain their cancerous state. In this study, we conduct a functional analysis of an unannotated RNA, lncRNA1, that is expressed specifically in breast cancer cells as opposed to non-cancerous breast cells. We hypothesize that this RNA is a lncRNA that acts as a miRNA decoy. Our findings present differential expression of the unannotated RNA in different breast cancer cell lines, as well as a potential miRNA, mir-570-3p, that could be sequestered and inactivated by lncRNA1. Our findings also suggest the non-coding nature of lncRNA1 through Open Reading Frame (ORF) analysis and present predictions of lncRNA1 secondary structure.

History

Date

2024-04-30

Academic Program

  • Biological Sciences

Advisor(s)

Ihab Younis

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