Investigating Effects of Site-Directed Mutagenesis on Activity and Expression of Endolysin in E.Coli BL21

Endolysins, also referred to as "enzybiotics," are thought to be a promising class of antibiotics generated from enzymes. Their main advantage is a high degree of specificity over traditional broad-spectrum antibiotics. Endolysins don't harm the good microbiota and have specialized bactericidal functions that could be modified by molecular engineering. Moreover, endolysins have additional benefits, including quick bacterial cell lysis, minimal risk of resistance, synergistic activity with various antibiotics, and the capacity to work well in biofilms and on mucosal surfaces. Therefore, investigating and narrowing down the mutations that can increase the interaction of endolysin with substrates of the bacterial cell wall, it can be used as a therapeutic tool to combat bacterial resistance.
For this reason, this project aims to utilize computational analysis of enzyme-substrate interactions to design point mutations using the site-directed mutagenesis GENEART System, which will increase the protein's lytic activity.