Profiling a ncRNA in Breast Cancer
The project focused on identifying and characterizing novel non-coding RNAs (ncRNAs) and miRNAs that are misregulated in different cancer subtypes, focusing on breast cancer cells. The project aims to explore the regulatory roles of ncRNAs in cancer cells and identify potential therapeutic targets for breast cancer treatment. RNA1 was used to profile ncRNAs in T47D, MCF7, PC3, HEK293, MDA-MB-468, and MDA-MB-231 cells. The results show that RNA1 is highly expressed in MCF7 and T47D, slightly in MDAMB468, but not in MDAMB231. The study showed that RNA1 binding sponges miRNAs. RNA I sponges hsa-miR-6973-30, leading to an increase in the inflammatory response. The miRNA hsa-miR-570-3p is a tumor suppressor targeting PD-L1 and inhibiting cancer cell growth via the PI3K/AKT/mTOR signaling pathway. It suppresses cell proliferation while promoting cell apoptosis. RNA I sponges hsa-miR-570-3p, affecting its tumor suppressor activity. Overall, this project provides important insights into the complex regulatory roles of ncRNAs in breast cancer and their potential as biomarkers and therapeutic targets.
- Biological Sciences