The Effect of Nuclear and Cytoplasmic hnRNPA2B1 Isoforms on Proliferation and Migration of Breast Cancer Cells

Breast cancer has the highest incidence rate and the second highest mortality rate among diagnosed malignant tumors (worldwide). In terms of the molecular mechanisms involved in breast cancer, there are many splicing factors that contribute to the aggressiveness of the cancer. One of them is hnRNPA2B1. Previous research shows that hnRNPA2B1 has a positive correlation with breast cancer cell proliferation but with respect to breast cancer metastasis, research studies differ in the conclusion- positive correlation or negative correlation. While hnRNPA2B1 is a splicing factor, it is itself alternatively spliced as well; out of the isoforms produced consequently, one that includes exon2 produces nuclear protein while the one excluding exon2 produces cytoplasmic protein. Since the previous research has focused on hnRNPA2B1 as a whole, here, we investigate the effect of each isoform of hnRNPA2B1 on both breast cancer cell proliferation and migration. Our results showed that transfection with hnRNPA2B1 AMO led to a reduction in the cell proliferation and cell migration of MDAMB231 cells.