Distance in pixels - 187 Distance in micrometer scale on SEM image - 10 Threshold_Type - cv2.ADAPTIVE_THRESH_GAUSSIAN_C Angle Step Size - 180 Median Blur - 3 Adaptive threshold - 11,2.5 Kernal Size - (3,3) Denoise - (2,2,2) Small Objects connectivity - 2 Small Holes connectivity - 1 Small Holes area_threshold - 55